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normal protein sequences are constrained by pressures to take care of needed structures and functions in a fancy cellular environment. nevertheless, vital cellular factors shaping protein sequences (for example interactions with mobile binding partners or Together with the proteostasis equipment) are often mysterious. To characterize functional constraints, it's been handy to determine mutational landscapes of proteins, which we outline listed here as the consequences on expansion of each probable solitary amino acid mutation within the protein, through deep mutational scanning (Boucher et al., 2016; Fowler and Fields, 2014). Deep mutational scanning reports have furnished insights into evolution of latest protein capabilities (McLaughlin et al.
The location on the DHFR gene is outlined in parenthesis in the primary column. Expression values corresponds on the mobile strain in the column heading.
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